Identifying Monoclonal Antibody Mutation Sites Using the Agilent 1290 Infinity II 2D-LC Solution with Q-TOF LC/MS
نویسندگان
چکیده
In recent years, 2D-LC has been highly promising for the detailed characterization and comparability assessment of protein biopharmaceuticals such as monoclonal antibodies. This Application Note describes the use of the Agilent 1290 Infinity II 2D-LC solution in the comparison of an infliximab originator and candidate biosimilar. RPLC×RPLC and SCX×RPLC peptide mapping revealed important differences that could be attributed to a double mutation in the heavy chain of the candidate biosimilar using quadrupole time-of-flight mass spectrometry.
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